Description
Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer). Source: human 293 cells. Endotoxin Level: <1 EU/µg. Biological Activity: measured by Noggin's ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is < 20 ng/ml in the presence of 30 ng/ml of human BMP-4. Formulation: Lyophilized from a 0.2 µM filtered, aqueous solution. Reconstitution: Supplied as a lyophilized solid. Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA